This is beneficial for restricting the amplification only to mRNA. You can even exclude these primers if you'd like to amplify mRNA plus the corresponding genomic DNA. Exon junction match
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Assistance Low complexity locations are some areas inside of a DNA sequence that have biased foundation compositions such as a extend of ACACACACACACACACACA. Internal hybridization oligo parameters
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Not like oligo(dT) primers, these primers will not be template particular and so are ideal for DNA synthesis applying Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates.
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Unlike oligo(dT) primers, these primers aren't template certain and are appropriate for click here DNA synthesis applying Klenow fragments with DNA templates or for cDNA synthesis making use of reverse transcriptase with mRNA templates.
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